Economic factors that affect the colgate brand

Economic factors that affect the colgate brand The overall economy of the country is showing a stable growth. Therefore this industry is also showing a growth of 10%. But the inflation is growing rapidly which is not a good sign for the new entrants. Change in the interest rate also affects the industry. Hence the new entrants are also threatened by the increasing interest rates. Economic factors do influence the bargaining power of buyers as inflation is the major factor that is affecting the consumers hard. Economic factors do not affect the availability of substitutes. But since inflation is growing, so it is hard for the poor people to switch to more modern products like washing powder, tooth paste. They would rather prefer oil to clean their clothes and use miswak to brush their teeth. Economic factors do have an affect on the bargaining power of suppliers as inflation is rising fast. Suppliers demand that they would provide the necessary raw material at a higher price. Exchange rate fluctuations also affect the cost of raw material. Economic factors do affect the rivalry among the competitors as all the players in the industry want to have cost leadership. The economic factors are favoring intense competition from the last five years. People have more money at their disposal. Also Pakistan is a consumption oriented society (Hiatt, Jeff.). Quantitative Strategic Planning Matrix (QSPM) STRATEGY 1 STRATEGY 2 Weights AS TAS AS TAS STRENGTHS Market leader in surface care with 90% market share 0.15 Good Leadership 0.06 Employee commitment 0.12 3 0.36 2 0.24 Cooling crystal patent 0.1 Accurate forecast with respect to demand 0.07 4 0.28 2 0.14 High EPS 0.08 3 0.24 2 0.16 Strong advertisement and promotional campaign 0.08 2 0.16 3 0.24 WEAKNESSES IT not used optimally 0.13 4 0.52 2 0.26 No ecommerce 0.1 3 0.3 2 0.26 No proper use of forward integration 0.05 2 0.1 3 0.15 They are not exporting their products to other countries 0.06 1 OPPORTUNITIES Exploring into new categories like shampoo, hand wash , body wash, shower gel 0.14 2 0.28 4 0.56 Rural population switching from miswak to toothpaste 0.06 Gap in tooth brush market 0.08 2 0.16 3 0.24 Implementation of ERP(SAP) 0.14 4 0.56 2 0.28 People becoming hygiene and beauty conscious 0.1 2 0.2 3 0.3 No layoffs 0.07 THREATS(T) Raw material and energy prices are increasing 0.12 3 0.36 2 0.24 Inflation in the country 0.07 3 0.21 2 0.14 Competitors re launching their products 0.1 2 0.2 3 0.3 Competitors increasing their marketing budgets 0.06 2 0.12 3 0.18 Unstable law and order conditions. 0.06 1 TOTAL 4.05 3.69 Rivalry among existing competitors Yes (+) No (-) The industry is growing rapidly. à ¢Ã‹â€ Ã… ¡ The industry is not cyclical with intermittent overcapacity. à ¢Ã‹â€ Ã… ¡ The fixed costs of the business are relatively low portion of total costs. à ¢Ã‹â€ Ã… ¡ There are significant product differences and brand identities between the competitors. à ¢Ã‹â€ Ã… ¡ The competitors are diversified rather than specialized. à ¢Ã‹â€ Ã… ¡ It would not be hard to get out of this business because there are no specialized skills and facilities or long-term contract commitments etc. à ¢Ã‹â€ Ã… ¡ My customers would incur significant costs in switching to a competitor. à ¢Ã‹â€ Ã… ¡ My product is complex and requires a detailed understanding on the part of my customer. à ¢Ã‹â€ Ã… ¡ My competitors are all of approximately the same size as I am. à ¢Ã‹â€ Ã… ¡ INTERPRETATION: Threat of competitors is high because the product is not unique as there are no product differences. Same products are available with all competitors like Unilever, Procter and Gamble, Shield, Oral B. Therefore rivalry is also increasing as demand in the economy has also increased for the last 5 to years. Majority of the competitors are MNCs which means that they have the necessary resource and skill (Hiatt, Jeff). Government Factors: Government regulations do have an affect on the rivalry among the competitors. Political factors: Political factors do not affect the rivalry among the competitors Economic factors Economic factors do affect the rivalry among the competitors as all the players in the industry want to have cost leadership. The economic factors are favoring intense competition from the last five years. People have more money at their disposal. Also Pakistan is a consumption oriented society (Hiatt, Jeff). Social trends Social trends have changed, buyer are more aware of their purchases. So the players in the industry are always trying hard to increase their market share (Hiatt, Jeff). Technological change Technology is helping the companies to gain a competitive advantage over their rivals. Also research and development plays an important role in this (Hiatt, Jeff). KEY SUCCESS ACTORS FOR COMPETIVE SUCCESS Low cost production efficiency. CP has plants installed that take only 30 minutes for the plant to shift from the production of one product to the other. High quality manufacturing of products with very few defects. A strong network of wholesale distributors. Gaining ample space on retailer shelves. (Examples Naheed, Aghas, Makro and other small shops.) Attractive packaging and styling. Good length of product lines. Attractive commercials and advertising. Good reputation in the industry along with favorable reputation with buyers. Market penetration Colgate can do market penetration by increasing its advertising, people always associate Colgate with tooth paste and have no awareness that it produces personal and surface care products as well. They should show their logo on all its products so that people know the producers and this will increase customer loyalty and also when people will come to know that Colgate is the producer then new customers will also buy the product (Hiatt, Jeff). They should also increase their promotion efforts by going to school and doing health awareness programs, also they should go to residential areas and make women aware of their surface care and fabric care products such as max, bonus and express etc Product development: Colgate can also do product development by introducing new features in their already existing brands. They can improve their tooth brush quality and features by introducing flexi tooth brushes for special oral care. Also they can introduce whitening features in their feature in their tooth paste; different colors of tooth paste can also be introduced to attract the kinds market. In their fabric care they can introduce in special features of protecting the color of the cloth while washing and also whitening enhancing surfs to give a good shine to while clothes (Hiatt, Jeff). develop systems to involve appropriate stakeholders in the introduction of change SIX THINGS REQUIRED TO EFFECTIVELY IMPLEMENTING A STRATEGY According to Porter, to implement a strategy effectively six things should be followed: Create a formal plan: Distributor Quantity Item Price Negotiation, Inventory Reporting, Stock Details, Payment Terms HR Module Inventory / Store Create a multifunctional team: To effectively implement ERP, Colgate must first hire an independent resource firm. Acquire licensing for SAP Implementation. Establish an independent IT unit for the monitoring of ERP. This department will coordinate with all the other departments (Robbins S, Mukerji). Communication of strategy: inside and outside: All employees in the company should be made aware of the new automated system. Each employee must be provided a minimal insight of SAP but those employees who have to work on SAP the most should ofcourse be provided with proper training course of SAP. These include: Factory Manager Manager accounts Warehouse Manager Distribution team Consumer insight department Outside the company the strategy to implement ERP should be communicated properly to all the local and foreign suppliers of Colgate Palmolive (Hagberg). Consistency over time: As Information Technology is progressing day by day, new changes in ERP are expected after certain time periods. Therefore it would become very important for Colgate to cope up with the changes in the software. For this purpose it would need to update it employees by providing training whenever any change takes place in SAP (Hagberg). Use proper measurements: Other measures, not just financial measures would be required to test whether the system is working properly. These measures include: Whether the system is accurately forecasting demand, sales, price changes Whether or not there are any deviations in the actual versus projected sales Effectiveness of the internal processes after the strategy implementation (Robbins S, Mukerji). Test the strategy: Ask employees and senior management in all departments whether they are satisfied with the new system of working. Talk to suppliers and distributors whether they want any more improvements in the new system of working (Hagberg). KEY DRIVING FORCES Industry growth: Now most of the people have knowledge about the different products and its advantages. The intense competition in industry and high demand enables the new entrants to compete in this market (Hagberg). Product innovation: Innovation in product, as Colgate has just introduced Max fresh in which the coolant crystals are present which none of the competitors has adopted yet. There is also room in innovation in washing detergents (Hagberg). Changing societal concerns, attitudes and lifestyle: Since social issues are really effecting the use of product just like the use of Miswak which is the Islamic mode of cleaning the teeth (Hagberg). Use of E-commerce and Internet: Usage of online ordering and maintaining the stock level through e-commerce can be driving force in the industry. By using that they can provide products to customer which is value addition for them (Hagberg). This report is purely based on the finding from the Colgate expert and the secondary data analysis, and the reason for writing the whole report is to find the obstacle / hindrance a business men face and how the organization can minimize it through implementing the different strategies and analysis. The whole report research draws attention to  the fact that any problem can be eliminate if proper investigation and consideration can be observed the success is easy to get, Pakistan market is mature market and the competition is tough here because most of the customer is price conscious and act like butterflies, so targeting this kind of customer is quite difficult, but on the other hand it can be managed if the you can understand the market effectively and efficiently (Robbins S, Mukerji). Hiatt, Jeff. HYPERLINK http://www.change-management.com/tutorial-definition-history.htmThe definition and history of change managementHYPERLINK http://www.change-management.com/tutorial-definition-history.htm. http://www.change-management.com/tutorial-definition-history.htm. Filicetti, John (August 20, 2007). HYPERLINK http://www.pmhut.com/pmo-and-project-management-dictionaryProject Management DictionaryHYPERLINK http://www.pmhut.com/pmo-and-project-management-dictionary Moore, E.R., (accessed 1 April, 2001), Creating Organizational Cultures: An Ethnographic Study, Eastern Academy of Management Virtual Proceedings, Moore, E.R., (accessed 1 April, 2001), Creating Organizational Cultures: An Ethnographic Study, Eastern Academy of Management Virtual Proceedings, , R. Ph.D. Heifetz, D.Ph., 2000 (accessed 4 April, 2001), Corporate HagbergCulture/Organisational Culture: UnderJerryding and Assessment, Robbins S, Mukerji D (1994). Managing organisations. Prentice Hall of Australia, McPhersons Printing Group, Australia. Sergiovanni, T.J. (1984) Educational Leadership, Leadership and excellence in schooling, Volume 41, Number 5, United States of America, page 4-13 Kent State University 2000 (accessed 28thMarch, 2001), Kent State University Cultural Self-Study Internal Communication of Change By Dagmar Recklies Circling the Pyramid Building Lasting Commitment to Change  Ã‚   (pdf-file) by Edmond Mellina What Makes a Good Change Agent? by Dagmar Recklies The Role of the Change Master From Change Agent to HYPERLINK http://www.themanager.org/Strategy/Change Master.htmHYPERLINK http://www.themanager.org/Strategy/Change Master.htmChange MasterHYPERLINK http://www.themanager.org/Strategy/Change Master.htm By Ruth Tearle Managing Change Definition and Phases in Change Processes by Oliver Recklies Problems in Managing Change by Oliver Recklies AuditNet.org provides a downloadable PDF of change management best practices. Fred Nickols wrote HYPERLINK http://home.att.net/~nickols/change.htmHYPERLINK http://home.att.net/~nickols/change.htmChange Management 101: A Primer.HYPERLINK http://home.att.net/~nickols/change.htm SearchCRM.com provides resources on handling change management following a CRM upgrade. Neglecting your change control process can kill an IT disaster recovery plan.

High Performance Liquid Chromatography Experiment

High Performance Liquid Chromatography Experiment INTRODUCTION Pharmaceutical Analysis may be defined as the application of analytical procedures used to determine the purity, safety and quality of drugs and chemicals. The term Pharmaceutical analysis is otherwise called quantitative pharmaceutical chemistry. Pharmaceutical analysis includes both qualitative and quantitative analysis of drugs and pharmaceutical substances starts from bulk drugs to the finished dosage forms. In the modern practice of medicine, the analytical methods are used in the analysis of chemical constituents found in human body whose altered concentrations during disease states serve as diagnostic aids and also used to analyze the medical agents and their metabolites found in biological system. Qualitative inorganic analysis seeks to establish the presence of given element or inorganic compound in a sample. Qualitative organic analysis seeks to establish the presence of a given functional group or organic compound in a sample. Quantitative Quantitative analysis seeks to establish the amount of a given element or compound in a sample. The term quality as applied to a drug product has been defined as the sum of all factors, which contribute directly or indirectly to the safety, effectiveness and reliability of the product. These properties are built into drug products through research and during process by procedures collectively referred to as Quality control. Quality control guarantees with in reasonable limits that a drug products Is free of impurities. Is physically and chemically stable Contains the amount of active ingredients as stated on the label and Provides optimal release of active ingredients when the product is administered. Most modern analytical chemistry is categorized by two different approaches such as analytical targets or analytical methods. INTRODUCTION FOR CHROMATOGRAPHY: High performance liquid chromatography is the process, which seperates mixture containing two or more components under high pressure. In this the stationary phase is packed in column one end of which is attached to a source of pressurized liquid mobile phase. High performance liquid chromatography is the fasted growing analytical technique for the analysis of drug. Its simplicity, high specificity and wide range of sensitivity makes its ideal for the analysis of many drugs in both dosage form and biologic fluids. HPLC is also known as High performance liquid chromatography. It is essential form column chromatography in which the stationary phase is consists of a small particles (3-5o µm) packing contained in a column with a small bore (2-5mm), one end of which is attached to source of pressurized liquid eluent(mobile phase). Different Types of Principles: According to the phases involved, HPLC can be classified into several types, which are as follows: Normal Phase Chromatography (NPC) Reverse Phase Chromatography (RPC) Liquid Solid Chromatography or adsorption HPLC Liquid Liquid Chromatography or Partition HPLC Ion exchange Chromatography or Ion exchange HPLC Size exclusion or gel permeation or steric exclusion HPLC 1. Normal Phase Chromatography (NPC): In normal phase chromatography, the stationary phase is more polar then the mobile phase, and the mobile phase is a mixture of organic solvents with out added water (e.g. isopropane with hexane) and the column packing is either an inorganic adsorbent (silica) are a polar bonded phase (cyanno, diol, amino) on a silica support. Sample retention in normal phase chromatography increases with the polarity of mobile phase decreases. They are eluted in the order of increasing polarities. 2. Reverse Phase Chromatography (RPC): In reverse-phase chromatography, the stationary phase is less polar than the mobile phase and the mobile phase is a mixture of organic and aqueous phase. Reverse-phase chromatography is typically more convenient and rugged than the other forms of liquid chromatography and is more likely to result in a satisfactory final separation. High performance RPC columns are efficient, stable and reproducible. In this, the solutes are eluted in the order of their decreasing polarities. These are prepared by treating the surface silanol group of site with an organic chloro silane reagent. INSTRUMENTATION: RECORDER SCHEMATIC DIAGRAM OF HPLC a. Pumps: Pumps are required to deliver a constant flow of mobile phase at pressures ranging from 1 550 bar pumps capable of pressure up to 6000 psi provide a wide range of flow rates of mobile phase, typically from 0.01-10ml min-1. Low flow rates (10-100à ¯Ã‚ Ã‚ ­l min-1) are used with micro bore columns, intermediate flow rates (0.5-2ml min-1) are used with conventional analytical HPLC columns, and fast flow rates are used for preparative or semi preparative columns and for slurry packing techniques. Mechanical pumps of the reciprocating piston type view a pulsating supply of mobile phase. A damping device is there fore required to smooth out the pulses so that excessive noise at high levels of sensitivity or low pressure does not detract from detection of small quantities of sample. This type of pump is mostly used. Dual piston reciprocating pumps produce an almost pulse free flow because the two pistons are carefully faced so that as one is filling the other is pumping. These pumps are more expensive than single piston pumps but are of benefit when using a flow sensitive detector such as ultraviolet or refractive index detector. b. Injection Systems: Injection ports are of two basic types, (A) those in which the sample with injected directly into the column and (B) those in which the sample is deposited before the column inlet and then swept by a valving action into the column by the mobile phase. c. Columns: HPLC columns are made of high quality stainless steel, polish internally to a mirror finish. Standard analytical columns are 4-5 mm internal diameter and 10-30 cm in length. Shorted columns (3-6 cm) containing a smaller particles size packing material (3 or 5 à ¯Ã‚ Ã‚ ­m) produce similar or better efficiencies, in terms of the number of theoretical plates (about 7000), that those of 20 cm columns containing 10 à ¯Ã‚ Ã‚ ­m irregular particles and are used an short analysis time and highest throughput of samples are required. Micro bore columns of 1-2 mm internal diameter and 10-25 cm in length have certain advantages of lower detection limits and lower consumption of solvent, the latter being important if expensive HPLC grade solvents are used. HPLC are also being carried out on the semi preparative scales by using columns of 7-10 mm or 20-40 mm internal diameter respectively. d. Detectors: The most widely used detectors for liquid chromatography are Detector Analytes Solvent Requirements Comments UV-Visible Any with chromophores UV-grade non UV absorbing solvents Has degree of selectivity and useful for many HPLC applications Fluorescence Fluorescent compounds UV-grade non UV absorbing solvents Highly selective and sensitive, often used to analyze derivitized compounds Refractive index Compounds with different RI than mobile phase Cannot run mobile phase gradients Limited sensitivity Conductivity Charged or polar compounds Mobile phase must be conducting Excellent for ion exchange compounds Electrochemical Readily oxidized or reduced compounds, specially biological samples Mobile phase must be conducting Very selective and sensitive Mass-Spectrometer Broad range compounds Must use volatile solvents or volatile buffers Highly sensitive. Many modes available. Needs trained person Theoretical principles of HPLC: a. Retention time: The time is required between the injection point and the peak maximum is called the retention time. It is denoted as the Rt. It is mainly useful for the qualitative analysis for the identification of compound. b. Capacity factor: It represents the molar ratio of the compound in the stationary phase and the mobile phase. It is independent of column length and mobile phase flow rate. It is denoted as the k. It should be kept 1-10. If k values are too low it is likely that the solutes may be adequately resolved and for high k values the analysis time is too long. It can be calculated by tr t0 k = - t0 tr = Retention time, t0 = Dead time. c. Tailing factor: Closer study of a chromatographic show that the Gaussian forms is usually not completely symmetrical. The graph spread out to a greater or lesser extent, forming a tail. It reduces the column plate number which intern influences the resolution. Tailing is mainly due to deteriorated column, overloading column, extra column-volumes, and incompatibility of sample with standard and/or mobile phase. Practically it can be calculated or determined at 10% of the total peak height. It must not be greater than 2.0 d. Resolution: The degree of separation of one component from another is described by the resolution. It is generally denoted by Rs. It is measured as the difference in retention time and the arithmetic mean of the two peak widths. tr2 tr1 Rs = 0.5(w1 + w2) tr2 = Retention time of first peak w1 = width of first peak tr1 = Retention time of second peak w2 = width of second peak e. Theoretical plates: It is important property of the column. It reflects its quality of separation and its ability to produce sharp, narrow peak and achieving good resolution of peak. N denotes it. 3500 X L (cm) Theoretical plates = - dp( µm) L = length of the column in cm, dp = diameter of the particle ( µm) It follows that if the exchange is fast and efficient, the theoretical plate will be small in size and there will be large number of plates in the column. f. Height equivalent to theoretical plate (HETP): Number of plates directly proportional to the column length (L) and inversely proportional to the diameter of the particles (dp). The value of H is a criterion for the quality of a column. Lower the HETP, higher is the efficiency of the column. Its value depends upon particle size, flow rate, viscosity of mobile phase. H = L/N L = Length of column, N = No. of theoretical plate HPLC method development: The wide variety of equipment, columns, eluent and operational parameters involved makes high performance liquid chromatography (HPLC) method development seem complex. The main objective of method development is to obtain a good separation with minimum time and effort. Based on the goal of separation, the method development is preceded. The steps involved are: Information on sample, define separation goals Need for special HPLC procedure, sample pretreatment, etc. Choose detector and detector settings Choose LC method, preliminary run; Estimate best separation conditions Optimize separation conditions Check for problems or requirement for special procedure Validation for release to routine laboratory The following must be considered when developing an HPLC method: Keep it simple Try the most common columns and stationary phases first Thoroughly investigate binary mobile phases before going on to ternary Think of the factors that are likely to be significant in achieving the desired resolution. Mobile phase composition, for example, is the most powerful way of optimizing selectivity whereas temperature has a minor effect and would only achieve small selectivity changes. pH will only significantly affect the retention of weak acids and bases. VALIDATION OF ANALYTICAL METHOD IN PHARMACEUTICAL ANALYSIS: Validation is documented evidence, which is completed to ensure that an analytical method is accurate, reproducible and robust over the specific range. The quality of the analytical data is a key factor in the success of a drug development program. The process of method development and validation has a direct impact on the quality of these data. Method validation: Method validation is the process to confirm that analytical procedure employed for a specific test is suitable for its intended use. Method needs to be validated or revalidated Before their introduction into routine use Whenever the conditions changes for which the method has been validated , e.g., instrument with different characteristics Whenever the method is changed, and the change is outside the original scope of the method. Depending on the use of the assay, different parameters will have to be measured during the assay validation. ICH and several regulatory bodies and Pharmacopoeia have published information on the validation of analytical procedures METHOD VALIDATION PARAMETERS: SPECIFICITY. ACCURACY. PRECISION. LINEARITY. ROBUSTNESS. SOLUTION STABILITY. The goal of the validation process is to challenge the method and determine the limit of allowed variability for the conditions needed to run the method. The following statistical parameters are to be determined to validate the developed method. Correlation coefficient(r): When the changes in one variable are associated or followed by changes in the other, it is called correlation. The numerical measure of correlation is called the coefficient of correlation and is defined by the relation. à ¯Ã‚ Ã¢â‚¬Å" (x x) (y -y) r = à ¢Ã‹â€ Ã… ¡ à ¯Ã‚ Ã¢â‚¬Å"(x -x) 2 à ¯Ã‚ Ã¢â‚¬Å"(y -y Regression equation: Regression equation= I + aC Y2 Y1 a = slope = X2 X1 I = Intercept = regression a C As a percentage of mean absorbance. 3. Standard Deviation: S = à ¢Ã‹â€ Ã… ¡ à ¯Ã‚ Ã¢â‚¬Å" (X- X!) 2/N 1 Where, X = observed values X! = Arithmetic mean = à ¯Ã‚ Ã¢â‚¬Å"X/N N = Number of deviations For practical interpretation it is more convenient to express S in terms of percent of the approximate average of the range of analysis is used in the calculation of S. This is called co-efficient of variation (C.V) or percent relative standard deviation (%RSD). C.V OR %RSD = 100* S/ X! Criteria for Validation of the Method CHARACTERISTICS ACCEPTABLE RANGE Specificity No Interference Accuracy Recovery (98-102%) Precision RSD Linearity Correlation Coefficient(r)>0.99 Range 80-120% Stability >24h or >12h DRUG PROFILE RIZATRIPTAN BENZOATE: Structure: Chemical name : N,N diethyl -5-(1H-1,2,4-triazol-1-1-ylmetyl)-1H Indole-3 Ethanamine monobenzoate Molecular Formula : C15H19N5.C6H5COOH Molecular weight : 391.47 Description: White crystalline powder Melting point: 178-1800C Solubility: Sparingly soluble in water and methanol Storage: Air tight container protect from light. Drug Category: Anti migraine drug THERAPEUTIC RATIONAL RIZATRIPTAN BENZOATE: CLINICAL PHARMACOLOGY: Mechanism of action: Rizatriptan binds with high affinity to human 5-HTIB and 5-HTID receptors leading to cranial blood vessel constriction. Pharmacokinetics: Absorption: Completely absorbed from GI tract, absolute bioavailability is 45% plasma peak concentration attained with in 1-1.5 hours (conventional tablet )or 1.6-2.5 hours (orally disintegrating tablet)after oral administration. Distribution: Crosses placenta and is distributed in to milk in animal, no studies in pregnant or nursing women. Metabolism: Metabolized principally via oxidative deamination by Mao-A to an inactive indole acetic acid metabolite Elimination: Excreted principally in urine(14% of dose as unchanged drug and 51 % a indole acetic acid metabolite Adverse effects: Dry mouth Dizziness Pain tightness/pressure in neck/throat/jaw. Nausea Chest pain Parasthesia Fatigue Dosage and administration: The dose range of Rizatriptan benzoate is 10-30mg orally once daily.Rizatriptan benzoate can be administer orally disintegrating tablet with out meals. LITERATURE REVIEW Sasmitha Kumar et al: has been developed UV spectroscopic method for estimation of Rizatriptan benzoate.The drug shows maximum absorption at 277 nm and 281 nm and obeys beer-lamberts law in the concentration of 0.5-20  µg/ml at 277 nm and 0.5-80  µg/ml at 281 nm respectively. The percentage recovery was found to be 97-100%. Madhukar et al; has been developed reverse phase high performance liquid chromatographic method for determination of Rizatriptan benzoate. The proposed method utilized column L1 inertsil ODS-3v, 250 nmx4.6 mm having particle size, 5 µm. The mobile phases were comprised of A, B of Acetonitrile and buffer pH 6.5 at UV detection 225 nm.The method shows recovery 96.64-97.71 Sachin jagthap et al; has been developed stability indicating reversed phase high performance liquid chromatographic method for the determination of Rizatriptan benzoate in bulk powder and in pharmaceutical formulations. The method utilizes c18 column having dimension 250mmx4.6 mm having particle size,5.0  µm using a mobile phase 0.01M sodium dihydrogen phosphate buffer: Methanol , at a flow rate 1ml/min at ambient temperature and detected at 225 nm.and the method was validated according to ICH guidelines Quizi zhang et al: has been developed, a high performance liquid chromatographic method for the determination of Rizatriptan benzoate in human plasma.using asingle step liqid liqid extraction with metyl tertiary butyl ether, the analytes separated usig amobile phase consisting of 0.05%v/v triehylamine in water adjusting ph 2.75 with 85% phosphoric acid and acetonitrile.fluroscence detection was performed at an excitation wavelength of 225 nm and an emission wavelength of 360 nm.The linearity for rizatriptan was within the concentration range of 0.5-50ng/ml. Rajendra Kumar et al: has been developed and validated stability a stability indicating high performance liquid chromatographic method for Rizatriptan benzoate.The force degradation studies were performed on bulk sample of Rizatriptan benzoate. The method utilizes a zorbax SB-CN column with dimension of 250 mmx4.6 mm, 5um column. The mobile phase consists of a mixture of aqueous potassium dihydrogen ortho phosphate (ph3.4), acetonitrile and methanol. Rauza bagh et al: has been developed a spectroscopic method for analysis of Rizatriptan benzoate in bulk and tablet dosage form. The Rizatriptan benzoate shows maximum absorbance at 225 nm. Beers law was obeyed in the concentration range of 1-10 µg/ml. AIM AND PLAN OF WORK The present aim is to develop a new simple and rapid analytical method to estimate the Rizatriptan benzoate The plan of the proposed work includes the following steps: To undertake solubility studies for analytical studies of Rosuvastatin calcium Develop initial chromatographic conditions. Setting up of initial chromatographic conditions for the assay of Rosuvastatin calcium Optimization of initial chromatographic conditions. Validation of the developed HPLC Analytical method according to ICH method validation parameters. EXPERIMENTAL NEW RP-HPLC METHOD FOR THE ESTIMATION OF RIZATRIPTAN BENZOATE IN TABLET DOSAGE FORM A simple reverse phase HPLC methods was developed for the determination of Rizatriptan benzoate in tablet dosage form. Zorbax Eclipse XBD C18 (250 cm ÃÆ'- 4.6 mm) column in isocratic mode with mobile phase Buffer ph 5.0: Methanol (80:20) was used and pH-3 adjusted with tri ethylamine. The flow rate was 1.0 ml/min and UV detection at 225nm. The retention time 3.0 min. The proposed method was also validated. EXPERIMENTAL 1. Instrumentation: Shimadzu LC-10A HPLC Vacuum pump Gelmon science Elico SL-164 double beam UV-Visible spectrophotometer Ultra sonicator 3.5L 100(pci) 2. Chemicals: Water HPLC grade Methanol HPLC grade (Merck) Potassium dihydrogen orthophosphate(AR Grade) Triethylamine (AR Grade) 5.1 OPTIMIZATION: 1. Selection of wavelength: After solubility study for the drug solvent was selected and appropriate concentration of Rizatriptan benzoate standards with solvent were prepared. The solution were then scanned by using doubl beam UV-Visible spectrophotometer the range between 200-400nm.The overlain spectra for the both drug were observed and maximum wavelength was finally selected. 2. Selection of mobile phase: To develop a prà ©cised and robust HPLC method for determination of Rizatriptan benzoate , its standard solution were injected in the HPLC system. After literature survey and solubility data different composition of mobile phase of different flow rates were employed in order to determine the best condition for effective separation of drugs. 3. Selection of column: Initially different C8 and C18 columns were tried for selected composition of mobile phase and quality of peaks were observed for the drugs. Finally the column was fixed upon the satisfactory results of various system suitability parameters such as column efficiency, retention time, tailing factor / peak asymmetry of the peaks. Other parameters such as flow rate, column temperature etc. were selected by varying its value up to certain levels and results were observed. The value at satisfactory results were obtained has been selected for the method. The final selection of chromatographic conditions as follows Optimized chromatographic conditions Preparation of Buffer ph 5.0: Dissove 2.76 gm of potassium dihydrogen orthophosphate in 1000ml of HPLC water plus 5.0 mlof Triethylamine. Mix and adjust PH 5.0 with orthophosporic acid. Filter with 0.45u nylon filter. Preparation of mobile phase: The mobile phase was prepared by mixing Buffer: Methanol (80:20). the solution was then filtered through 0.45ÃŽÂ ¼m membrane filter and sonicated. Preparation of standard stock solution: Standard solution of the pure drug was prepared by dissolving 73.0 mg of Rizatriptan benzoate in 100ml volumetric flask. The drugs were dissolved by using mobile phase as a diluent. Add about 50ml of diluent and sonicate to dissolve. Make up the volume with diluent. Mix well. Further dilute 5.0ml of the above solution to 250ml with diluent, mix well. Preparation of sample solution: Weight and transfer 10 intact tablet in into a100ml volumetric flask. Add about 50ml of diluent and sonicate for 15 min and make up the volume with diluent. Mix well, filter through 25 mm 0.45 u nylon , discard 4ml filtrate. Further dilute 5ml of the solution to 250 ml with diluent and mix well. CONCLUSION The evaluation of obtained values suggests that the proposed HPLC methods provide simple, precise, rapid and robust quantitative analytical method for determination of Rizatriptan benzoate in tablet dosage form. The mobile phase is simple to prepare and economical. After validating proposed method as per ICH guidelines and correlating obtained values with the standard values, satisfactory results were obtained. Hence, the method can be easily and conveniently adopted for routine estimation of Rizatriptan benzoate in tablet dosage form.

Anorexia Nervosa and Obsessive Compulsive Disorder Essays -- Essays on

Anorexia Nervosa and Obsessive Compulsive Disorder It has long been recognized that there are similarities between Obsessive Compulsive Disorder (OCD) and Anorexia Nervosa. These similarities lie in the symptoms of the disorder. Many patients of both diseases report intrusive, fearful thoughts, a compulsive need to perform rituals, and an obsession with maintaining these rituals. In the case of anorexia nervosa these behaviors center on food and thinness whereas in OCD they are of a more general type. Past research has indicated that there is a higher prevalence rate for OCD among anorexia nervosa patients then in the normal population. The lifetime prevalence for OCD has been found to be around 3%. Conversely, the comorbidity rate for OCD and anorexia has been reported to be between 6% and 33%! This leads one to hypothesis that there may be a relationship between OCD and anorexia nervosa. It is not clear yet, based on the present research, what this relationship is. But, there are many studies out there that try to examine the relationship as well as its underlying causes. In this literature review some of this research will be examined and some preliminary conclusions will be drawn. Finally, further research to explore this relationship will be proposed. Anorexia Nervosa Anorexia nervosa is a specific disorder defined in DSM IV. Several classifications must be met for a diagnosis of anorexia to be made. There must be a refusal by the patient to maintain a healthy body weight for their age and height. This behavior must eventually lead to a weight loss in which the body weight falls to less then 85% of the persons ideal body weight. Or the patient can refuse to gain any weight during periods of growth. In wome... ...urther studies are conducted that lead to a better connection between the two disorders, sufferers can be treated more efficiently. Reference Gee, Rebecca, Telew, Nicholas. Obsessive Compulsive Disorder and anorexia nervosa in a high school athlete: A case report. Journal of Athletic Training. 1999: 34:375-378 Han L, Nielsen D, Rosenthal N, Jefferson K, Kaye W, Murphy D, Altemus M, Humphries J, Cassano G, Rotondo A, Virkkunen M, Linnoila M, & Goldman D. No coding varient of the tryptophan hydroxylase gene detected in seasonal affective disoder, obsessive-compulsive disorder, anorexia nervosa, and alcoholism. Biological Psychiatry. 1999:45:615-619. Thiel A, Zurger M, Jacoby G, & Shussler G. Thirty month outcome in patients with anorexia or bulimia nervosa and concomitant obsessive-compulsive disorder. The American Journal of Psychiatry. 1998: 155:244-249.

Corporate Social Responsibility with HIV / AIDS Drugs :: Social Responsibility Essays

â€Å"Each year, just three diseases kill 5.4 million people worldwide. Malaria kills at least one million, mainly in developing countries, with 90% of the deaths in Africa. Tuberculosis causes 1.9 million deaths a year, almost all of them in developing countries, where resistance to the five major anti-tubercular drugs is spreading. Some 400,000 of the tuberculosis victims are also HIV positive. The yearly death toll for HIV/Aids is almost 2.5 million, with about 1.8 million concentrated in the Sub-Saharan Africa† The Times Higher 20/07/01 Assess the responsibilities of the pharmaceutical companies for providing low cost drugs to the poor people in the developing countries. There is no generally accepted definition for corporate social responsibility (CSR), although various theorists have attempted to surmise one. One useful definition is provided by Archie Carroll (1979): ‘The social responsibility of business encompasses the economic, legal, ethical and discretionary expectations that society has of organisations at a given point in time’ Another definition is offered by Kok et al: ‘The obligation of the firm to use its resources in ways to benefit society, through committed participation as a member of society, taking into account the society at large and improving the welfare of society at large independent of direct gains of the company.’ An alternative definition by Frederick et al (1988): ‘business and society are interdependent and co-exist, with business using society's resources and, in turn, fulfilling economic needs and changing social goals Thus, business and society are bound by a reciprocal "social contract" which is seen as the "core idea of corporate social responsibility† These definitions encapsulate the notion that business has a responsibility to society as a whole, aside from the basic profit maximising ideas dictated by traditional free-market theory. in direct contrast from the classical economic sense of increasing shareholder wealth (Friedman, 1970) that it is a ‘fundamentally a subversive doctrine’. In the pharmaceutical industry has ia system that produces drugs in two categories. Category A would include drugs for conditions like

Creative Story: The Dark House :: essays research papers

Creative Story: The Dark House by Tom-Inge Earlier today, John and I were taking a walk in the outskirts of the town, when we saw an old house on top of a hill. I wanted to take a closer look at it, but John looked a little spooked, and mumbled something about not wanting to go there. I convinced him that there was nothing to be afraid of, it's just an old house which haven't been occupied for a while, though I wasn't sure whether someone really was living there. We walked up the driveway, up to the house. It was huge. The place looked deserted, the grass wasn't mowed for ages, and everything was a mess. John chew nails like crazy, but I still wanted to check this place out. The door was made of wood, and looked like it could fall apart any minute. I tried to turn the door knob, but it fell off as soon as I touched it. The knob rolled down the steps and kept on rolling down the driveway, and stopped by the fence. That didn't make John feel any better at all. He looked like he had seen a ghost. I took a deep breath, and pushed the door. It wouldn't budge an inch.  « Help me out », I said, but John kept chewing his nails. God knows why, there was nothing to be scared of. Or was it? I took a step backwards, and then kicked the door. It slowly slid open, and I went inside.  «Wow! What a huge mansion we've got here! » John took a peek into the house, but didn't dare go inside. There was a huge hall in the middle of the house, with staircases to my left and right. I went right ahead, towards a glass door in front of me. It wasn't locked, so I carefully turned the knob, and the door opened. Suddenly I felt someone touch my shoulder. I turned around immediately to see, but there was nobody there.  «Probably just my imagination », I said to myself, and went on into the room. It was a lounge, with some impressive furniture, antiques and other objects. It was a lot to look at, and I yelled for John. He didn't reply, so I went to check on him. All I found outside the house was a shoe, and his necklace. I couldn't quite understand what was going on. If he'd run away, at least he'd taken his belongings with him. The door slammed shut behind me, and my heart stopped for a second or two. Creative Story: The Dark House :: essays research papers Creative Story: The Dark House by Tom-Inge Earlier today, John and I were taking a walk in the outskirts of the town, when we saw an old house on top of a hill. I wanted to take a closer look at it, but John looked a little spooked, and mumbled something about not wanting to go there. I convinced him that there was nothing to be afraid of, it's just an old house which haven't been occupied for a while, though I wasn't sure whether someone really was living there. We walked up the driveway, up to the house. It was huge. The place looked deserted, the grass wasn't mowed for ages, and everything was a mess. John chew nails like crazy, but I still wanted to check this place out. The door was made of wood, and looked like it could fall apart any minute. I tried to turn the door knob, but it fell off as soon as I touched it. The knob rolled down the steps and kept on rolling down the driveway, and stopped by the fence. That didn't make John feel any better at all. He looked like he had seen a ghost. I took a deep breath, and pushed the door. It wouldn't budge an inch.  « Help me out », I said, but John kept chewing his nails. God knows why, there was nothing to be scared of. Or was it? I took a step backwards, and then kicked the door. It slowly slid open, and I went inside.  «Wow! What a huge mansion we've got here! » John took a peek into the house, but didn't dare go inside. There was a huge hall in the middle of the house, with staircases to my left and right. I went right ahead, towards a glass door in front of me. It wasn't locked, so I carefully turned the knob, and the door opened. Suddenly I felt someone touch my shoulder. I turned around immediately to see, but there was nobody there.  «Probably just my imagination », I said to myself, and went on into the room. It was a lounge, with some impressive furniture, antiques and other objects. It was a lot to look at, and I yelled for John. He didn't reply, so I went to check on him. All I found outside the house was a shoe, and his necklace. I couldn't quite understand what was going on. If he'd run away, at least he'd taken his belongings with him. The door slammed shut behind me, and my heart stopped for a second or two.

Rekj

Additionally, if the slower receipt of payments impacts the organization's ability to quickly pay its own operational expenses then you might also see an increase in the accounts payable liability account. Conversely, on the Income Statement, one would expect to see an increase in the patient revenue account since the deal was that the NCO had longer to pay in trade for a HIGHER reimbursement rate. Also, on the Income Statement as the receivables account increases then you would also see an increase in the Provision for Doubtful Accounts.How might negotiating an NCO contract affect financial statements? The accounts that will be impacted on the Balance Sheet (which was the nature Of my question) are as follows. How does the Provision for Doubtful Accounts work? Posted by ROBERT ADAMS atlas 16, 2015, 8:09 PM If a hospital such as FCC renegotiated an NCO contract that allowed the NCO to take longer to pay in exchange for a higher reimbursement rate, this would increase patient revenue on the Income Statement because the NCO is paying at a higher rate.However, since the NCO has longer to pay then the Patient Accounts Receivable (A/R) on the Balance Sheet would also increase. As this A/R account increases, the amount booked as Provision for Doubtful Accounts would also increase since this expense is often simply a percentage of the A/R account. OR a more real world response: In the case of FCC, they treat the Provision of Doubtful Accounts as an expense account. This is an account that is used to ‘book† the projected losses associated with carrying a receivables account on the Balance Sheet for patient revenue.You might ask why an organization would need to do this. Well anytime you have a receivables account there is some likelihood that some of that account will go uncollected. Generally, an organization can use historical data to project what percentage of a receivable account might not be collected. Regarding this assignment, FCC renegotiated a prim ary managed care contract which allowed the NCO longer to pay in exchange for a higher reimbursement rate. The impact of the NCO having longer to pay means that the receivables account would grow.If FCC applies its historical percentage for uncorrectable to a growing receivables account then the Provision for Doubtful Accounts would naturally increase as well. How does purchasing extra inventory change financial statements? As FCC purchases inventory it is placed on the Balance Sheet as an asset. It is VERY important for you all to understand that the supplies expense will not increase on the Income Statement UNTIL the supplies are actually used by FCC. For instance, if FCC purchased $1 of extra inventory this loud be listed as an asset on the Balance Sheet.

Donne as a Metaphysical Poet Essay

Introduction During the past forty years there have been two major theories of language learning by children. But there are two major schools of thought known as, ‘Behaviorists’ and ‘Mentalists’. One school is of the view that language learning is entirely the product of experience and that our environment affects all of us. Others have suggested that everybody has an innate language learning mechanism. Let us discovery with the help of these two schools of thought that how do children acquire their mother tongue. How do they grow up linguistically and learn to handle the stylistics varieties of their mother tongue? How much of the linguistics system they are born with and how much do they discover from their exposure to language? a) The Behaviorist School B.F. Skinner and his followers are known as behaviorist. According to them language learning is process known as operant conditioning. Conditioned Behavior is behavior which is the result of repeated training. Operant means that it is voluntary behavior, it is result of learner’s own free will, and it is not forced by any outside person or thing. The learner demonstrates the new behavior first as a response to a system of reward or punishment, and finally as an automatic response. In order to prove their theory they conducted an experiment. EXPERIMENT They put a rat in a box containing a bar. If it presses a bar, it is rewarded with a pellet of food. Nothing forces it to press the bar. The first time it probably does so accidentally. When the rat finds that the food arrives, it presses the bar again. Eventually it finds that if it is hungry it can obtain food by pressing the bar. Then task is made more difficult. The rat only gets rewarded if it presses the bar while a light is flashing. At first rat is puzzled. Eventually it learns the trick. Then the task is made more difficult again. This time the rat only receives food if it presses the bar a certain number of times. After initial confusion it learns to do this also. And so on, and so on. Operant condition can be summarized thus STIMULUS RESPONSE REINFORCEMENT REPETITION In operant conditioned, reinforcement plays a vital role. There are two kinds of reinforcement: A) Positive Reinforcement Praise and rewards are positive reinforcement. Experiments have shown that positive reinforcement works much better in bringing about good learning. B) Negative Reinforcement Rebukes and punishments are negative reinforcement. The behaviorists also claim that we learn language by imitation and association. For example, a young child hears the word â€Å"water† with the actual thing. He then makes this sound himself, imitating what he has heard. His parents are pleased that he has learnt another word and so his response is reinforced. The thoughts of behaviorist school can well be understood according to following tree diagram. The Behaviorist School Language learning is Positive Imitation Operant conditioning and and Negative Association Reinforcement Noam Chomsky explicitly rejects the behaviorists’ position that language should be thought of as verbal behavior, arguing that it should be thought of as knowledge held by those who use language. Chomsky suggests that the learner of any language has an inbuilt learning capacity for language that enables each learner to construct a kind of personal theory or set of rules about the language based on very limited exposure to language. b) The Mentalist School Chomsky and his mentalist followers claim that a child learns his first language through cognitive learning. They claim that language is governed by rules, and is not a haphazard thing, as Skinner and his followers would claim. According to Chomsky, the child is born with a mental capacity for working out the underlying system to the jumble of sounds which he hears. He constructs his own grammar’ and imposes it on all the sounds reaching his brain. This mental grammar is part of his cognitive framework, and nothing he hears is stored in his brain until he has matched it against what he already knows and found a ‘correct’ place for it within this framework. Chomsky argues that language is so complex that it is almost incredible that it can be acquired by a child in so short a time. He says that a child is born with some innate mental capacity which helps the child to process all the language which he hears. This is called the Language Acquisition Device, and he saws it as comprising a special area of the brain whose only function was the processing of language. This function, he argues, is quite separate from any other mental capacity which the child has. When Chomsky talks about ‘rules’, he means the unconscious rules in a child’s mind these rules enables him to make grammatical sentences in his own language. Chomsky does not mean that a child can describes these rules explicitly. For example, a four or five year old child can produce a sentence like I have done my work; he can do that because he has a ‘mental grammar’ which enables him to form correct present perfect structures and also to use such structures in the right and appropriate situations. But he is unable to define the formation of present perfect tense. The thoughts of Mentalists can well be understood with the help of the following tree diagram. The Mentalists School Language learning Input Mental grammar Is an (own rules) Innate ability LAD Grammatical Output sentences Both the schools have said significant things, yet neither is perfect. The mentalists’ emphasis on the rule-learning is over enthusiastic, and the behaviorists’ rejection of meaning is entirely unjust. Language acquisition seems to be a process both of analogy and application, both nature and nurture. The differences between the empiricists approach and that of the rationalist can be summarized in the following manner: BEHAVIOURISTS APPROACH MENTALIST APPROACH Conclusion This comparative study makes one thing clear: nature and nurture, analogy and application, practice and exposure are important. Innate potentialities lay down the framework. Within this framework, there is wide variation depending on the environment. The kind of language that children ultimately grow into shaped by the culture-based responses of the family, if not in a way that can be called imitation, then at least in terms of things the child chooses to do with its language. But we should be wary of the idea that all children experience the same practices and follow the same development path as they grow into their language. Having been exposed to a small number of utterances, the child begins to extract the principles underlying the utterances and compose new utterances of his own. This is the way every child grammar to communicate in an intelligent manner. He makes mistakes and produces ungrammatical sentences. His elders correct him; he feeds the information into his mini-grammar, modifies some of the rules, and again produces new utterances. In a period of about four years, he is able to master and internalize all the essential rules of language. This is a proof that a child’s own rules of grammar are more important to him than mere imitation.